Paper chromatography - Principle, procedure, Applications.
Chromatography has numerous applications in biological and chemical fields. It is widely used in biochemical research for the separation and identification of chemical compounds of biological origin. In the petroleum industry the technique is employed to analyze complex mixtures of hydrocarbons.
Thin-layer chromatography is used to separate components of a plant extract, illustrating the experiment with plant pigments which gave chromatography its name Chromatography is a laboratory technique for the separation of a mixture.
High performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of pharmaceutical and biological samples.
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Chromatography is a method in which the components of a mixture are separated based on their differential interactions with two chemical or physical phases: a mobile phase and a stationary phase. 1-4 The basic components and operation of a typical chromatographic system are illustrated in Fig. 1.1.The mobile phase travels through the system and carries sample components with it once the sample.
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